2.16
High content imaging (HCI) is a high-throughput, automated microscope-based technique. With several fluorescent dyes, HCI assays can provide information on multiple properties of individual cells. Conventional HCI assays carried out on monolayer cell cultures are limited in their capability in predicting drug toxicity and efficacy in vivo due to the highly limited properties of 2D cell cultures to form tissue-like structures, while HCI on 3D cell cultures, combining the advantages of both 3D cell culture and high content screening, provides more liable data with multiple benefits.